Intranasal Peptide Administration Protocol

Confirm that the peptide being studied is appropriate for intranasal delivery. Peptides commonly administered intranasally in research include Selank, Semax, DSIP, and oxytocin — these have established nasal bioavailability data in the literature. Larger peptides (>5 kDa) generally have poor nasal absorption without permeation enhancers.

Use a sterile metered-dose nasal spray bottle calibrated to deliver a known volume per actuation (typically 0.1 mL per spray). Prime the spray device by pumping 3-5 times until a fine, consistent mist is produced. Discard any priming volume.

Determine the number of sprays needed based on your peptide concentration and target dose. For example, if your reconstituted peptide is 1 mg/mL and the target dose is 200 mcg, and each spray delivers 0.1 mL, you need 2 sprays (0.1 mL x 2 = 0.2 mL = 200 mcg). Split the dose equally between nostrils when possible.

Ensure nasal passages are clear before administration. Gentle saline irrigation or blowing the nose 5-10 minutes prior can improve mucosal contact. The nasal mucosa should be moist but not congested for optimal peptide absorption.

Tilt the head slightly forward (not back). Insert the spray nozzle just inside one nostril, angled slightly toward the outer wall (lateral direction, away from the septum). Close the opposite nostril with a finger. Actuate one spray while inhaling gently through the nose. Repeat in the other nostril if splitting the dose. Avoid sniffing forcefully — a gentle inhalation keeps the solution on the nasal mucosa rather than draining to the throat.

Remain upright with the head in a neutral position for 2-3 minutes after administration. Avoid blowing the nose for at least 15 minutes. Avoid eating, drinking, or lying down for 10-15 minutes to minimize solution clearance from the nasal cavity.

Record the peptide name, dose, number of sprays per nostril, time of administration, and any observations. Store the nasal spray bottle at 2-8°C between uses. Note the reconstitution date — intranasal peptide solutions typically remain stable for 14-21 days when refrigerated.