Analytical Methods
HPLC
Definition
High-Performance Liquid Chromatography (HPLC) is an analytical technique used to separate, identify, and quantify components in a mixture. In peptide research, HPLC is the gold standard for determining peptide purity by separating the target peptide from synthesis byproducts, truncated sequences, and other impurities. The resulting chromatogram shows peaks corresponding to each component, with the main peak area percentage indicating purity.